Download UN‑SCAN‑IT|UN‑SCAN‑IT gel Demo for Windows
Click Here to Download UN‑SCAN‑IT|UN‑SCAN‑IT gel Demo for Windows
- Click on the link above
- Save the download file Demozip.exe to its own directory or folder
- Run the file Demozip.exe to extract the demo files, and start the install program setup.exe
- Run the file Demo.exe from the Run Menu or Programs Menu
Note: The UN‑SCAN‑IT and UN‑SCAN‑IT gel software are included in this demo. The demo should be intuitive, and there are help buttons available. Additionally, instructions are located in the file readme.txt and a demo video and tutorial can be found below. Please let us know if you have any questions or difficulties using the demo. Tel: 1-801-377-6978 (USA) | Email: info@silkscientific.com
Download UN‑SCAN‑IT|UN‑SCAN‑IT gel Demo for Macintosh
Click Here to Download UN‑SCAN‑IT|UN‑SCAN‑IT gel Demo for Macintosh
- Click on the link above
- Save the download file UN‑SCAN‑IT_Demo.dmg to your Mac desktop
- Open the disk image and drag the UN‑SCAN‑IT Demo folder to your hard disk
- Double-click the UN‑SCAN‑IT Demo Icon in the UN‑SCAN‑IT Demo folder to run the program
Note: The UN‑SCAN‑IT and UN‑SCAN‑IT gel software are included in this demo. The demo should be intuitive, and there are help buttons available. Additionally, instructions are located in the file readme.txt and a demo video and tutorial can be found below. Please let us know if you have any questions or difficulties using the demo. Tel: 1-801-377-6978 (USA) | Email: info@silkscientific.com
Demo Videos & Tutorials
Thank you for using the UN‑SCAN‑IT and UN‑SCAN‑IT gel Demo software. Although the demo program should be intuitive, and does contain context sensitive help information, please let us know if you have any questions regarding the use of this demo program or any of the UN‑SCAN‑IT or UN‑SCAN‑IT gel features. If you would like us to offer direct assistance for your particular sample image, please email a copy of the image to info@silkscientific.com.
Demo Video | Graph Digitizing
Click here for Windows format video (.wmv)
Click here for Macintosh format video (.mov)
Demo Tutorial | Graph Digitizing
The UN‑SCAN‑IT software simply and easily converts pixel locations from a scanned hard copy graph into useful (x,y) data. The data can be used to analyze the graph, integrate peak areas, re-scale graphs, remove grid lines, smooth data, prepare publication quality graphs, etc. The (x,y) data can also be exported into spreadsheet, graphics, or data analysis software.
Digitizing the Sample Graph
After the introductory demo screen has displayed, select Digitize a Graph from the welcome screen (or select Digitize from the main menu). Select any of the displayed sample images. Once the sample scanned image has loaded, the Scan Mode and Setup settings will appear (descriptions of each of the options can be accessed by clicking the Help button). Although you can adjust the Scan Mode and Setup selections, the default settings are generally correct for the sample image. Click OK to accept these settings.
You will be prompted to define the scale and other setup parameters on the graph by dragging the setup icons to their corresponding graphical locations. You can drag the icons by clicking the left mouse button and dragging the icon to the desired location (hold mouse and arrow keys for fine movement). The zoom box in the upper right corner of the digitizing screen is useful for obtaining precise alignments during the axis setup process (the zoom window is for visual alignment purposes only, and all crosshair movements should be performed on the main image). After finishing the setup, click the Digitize button to automatically digitize the image. You can toggle between automatic and manual mode during the digitizing process by clicking the Pause and Continue buttons, respectively. When the digitizing process is paused (manual mode), you can access and change settings in the Options menu or add additional points manually. When the digitizing process is complete, you can accept the digitized data values and return to the main menu (or digitize additional data points).
Digitizing Your Own Image (Imagecode | Passcode)
The demo program will also allow you to test digitize one of your own images. You should scan and save your image "right side up" in a standard image format (JPG, TIF, BMP, PICT, etc.). The image should generally be saved in Line Art mode (otherwise known as black and white drawing or 1-bit mode). In order to digitize your own image, load the image into the Digitize (x,y) Graph Image portion of the demo software. The program will then display a unique Imagecode. Telephone or Email us with that Imagecode, and we will provide the corresponding Passcode that allows that image to be read. The Imagecode and Passcode are unique to each image, and are date sensitive.
Although the steps involved in digitizing your own image are generally similar to those described for the sample graph, each image can present its own potential difficulties. We would suggest that you contact us and/or e-mail us a copy of your sample image file if you would like assistance digitizing your own image.
Graphing the Data
To graph, analyze, or edit the data points from within UN‑SCAN‑IT, select Graph (x,y) Data from the drop-down Graph menu contained on the main page. The graphics buttons at the left of the graph allow you to integrate peak areas, transform the data resolution (increase or decrease the data spacing), smooth data, edit data, take derivatives, etc. Additional graphing choices are contained in the Options menu.
Integrating Peak Areas - To integrate a peak area, you must move both graphic cursors to their desired locations. The Left and Right Cursors can be selected by clicking on the appropriate buttons at the bottom of the graph. The cursors can be moved with the mouse or arrow keys. The (x,y) value of the data point at the active cursor appears at the upper left corner of the screen. Click the Area button to calculate the peak area between the cursors. Click anywhere on the graph to return to normal graphing mode.
To return to the main menu, select Exit.
Saving the Data
To save the (x,y) data for further analysis or graphing using other software programs… select Save (x,y) Data from the drop-down File menu contained on the main page. UN‑SCAN‑IT can save the data in ASCII text format (tab or comma delimited), which can be read by most spreadsheet, graphics, and data analysis programs. In addition, the data can be copied to the clipboard (select Copy to Clipboard from the drop-down File menu on the main page), and pasted directly into other software.
Demo Video | Gel Analysis
Click here for Windows format video (.wmv)
Click here for Macintosh format video (.mov)
Demo Tutorial | Gel Analysis
The UN‑SCAN‑IT gel software contains all of the (x,y) graphical digitizing features of regular UN‑SCAN‑IT, as well as features for analyzing electrophoresis gels and TLC plates. The digitized data can be used to determine optical densities, relative concentrations, band locations, and molecular weights.
Analyzing the Sample Gel
After the introductory demo screen has displayed, select Digitize Gel Image from the main Digitize menu. Select any of the displayed sample images. These gel images were scanned and saved in 8-bit grayscale mode. Once the image has loaded, the Gel Analysis Mode and Setup settings will appear (descriptions of each of the options can be accessed by clicking the Help button). Although you can adjust the Gel Analysis Mode and Setup selections, the default settings are generally correct for the sample image. Click OK to accept these settings.
Lane Analysis
You will be prompted to define the lanes of interest; to do so, click on the upper left-hand and corner of the desired lane and drag the mouse cursor to the lane's lower right-hand corner. Additional lanes can be drawn using the steps above, or the lane(s) can be copied (see Options menu) to ensure equally sized lanes. To relocate any lane, move the crosshair until the desired lane is highlighted, then click and drag the lane to its proper location. Additionally, the lane positions and sizes can all be adjusted by selecting Align Regions of Interest from the Options menu or by editing the values in the grid on the right side of the screen. When all of the lanes have been defined, click the Digitize button.
A density profile plot for each lane will appear on the screen. Although many of the peaks in the profile are found automatically, you can add or delete peaks by clicking the appropriate button. The peak baseline locations can be adjusted by using the mouse to drag the endpoints of the peak baseline to the desired position (the adjustments can be seen on the image at the right of the screen). To integrate the optical intensity area for each peak in the lane, click the Quantify Peaks button. Only the data above the baseline/background will be included in the area calculation, and this value will later be displayed as Pixel Total. After Quantify Peaks and Continue are selected, the program will then repeat the density profile process for the next lane.
When the analysis of all of the lanes is complete, the band positions and locations will be shown on the gel image and the band parameters displayed in the grid at the bottom of the screen. To calculate the molecular weight, double click the bottom grid Mol Weight column for the desired segment of known value and enter the value. Entering the value for two or more segments will result in molecular weight being calculated for the remaining segments (based on a standard least squares fit of Position vs. Log MW).
Segment and Dot Blot Analysis
You will be prompted to define the segments of interest; to do so, click on the upper left-hand corner of the desired segment and drag the mouse cursor to the segment's lower right-hand corner. Additional segments can be drawn using the steps above, or the segment can be copied (see Options menu) to ensure equally sized boxes. To relocate any segment box, move the crosshair until the desired segment is highlighted, then click and drag the box to its proper location. Additionally, the segment positions and sizes can all be adjusted by selecting Align Regions of Interest from the Options menu or by editing the values in the grid on the right side of the screen. When all of the segments have been defined, click Digitize in the lower right corner of the screen.
Background Corrections
Although the background is automatically subtracted when digitizing lanes (using the density profile), the segment analysis mode simply sums the pixel values for all pixels contained in the segment box. Therefore, a non-zero background will result in higher pixel totals due to additional background values being included in the sum. Background interference can be reduced or eliminated by selecting the Background Correction feature during the Setup process.
When the analysis of all of the segments is complete, the band positions and locations will be shown on the gel image and the band parameters displayed in the grid at the bottom of the screen. To calculate the molecular weight, double click the bottom grid Mol Weight column for the desired segment of known value and enter the value. Entering the value for two or more segments will result in molecular weight being calculated for the remaining segments (based on a standard least squares fit of Position vs. Log MW).
Concentration and Molecular Weight Calibration Curves
A calibration curve for both the Concentration and Molecular Weight values for each band can be displayed by clicking the Show Concentrations or Show Mol. Weights buttons on the toolbar of the results screen. In order to properly display the calibration curve, at least two concentration or molecular weight values must be entered in the lower spreadsheet for the standard or marker bands. The software will then use a standard least squares fit to estimate the values for the remaining bands.
Analyzing Your Own Image (Imagecode | Passcode)
The demo program will also allow you to analyze one of your own images. You should scan and save your image "right side up" in a standard grayscale image format (JPG, TIF, BMP, PICT, etc.). In order to analyze your own image, load the image into the Digitize Gel Image portion of the demo software. The program will then display a unique Imagecode. Telephone or E-Mail us with that Imagecode, and we will provide the corresponding Passcode that allows your image to be read. The Imagecode and Passcode are unique to each image, and are date sensitive.
Although the steps involved in analyzing your own image are generally similar to those described for the sample gel, each image can present its own potential difficulties. We would suggest that you contact us and/or e-mail us a copy of your sample gel if you would like additional assistance analyzing your own image.
Saving the Data
To save the gel data for further analysis, calibration, or graphing using other software programs… Select Save gel Data from the drop-down File menu contained on the main page. UN‑SCAN‑IT gel can save the data in ASCII text format (tab or comma delimited), which can be read by most spreadsheet, data analysis, and graphics programs. In addition, the data can be copied to the clipboard (select Copy to Clipboard from the drop-down File menu), and pasted directly into other programs.
Removing the UN‑SCAN‑IT|UN‑SCAN‑IT gel Demo
Windows - To remove the Demo, use the Windows Add/Remove Programs feature from the Windows Control Panel.
Macintosh - To remove the Demo, simply drag the entire UN‑SCAN‑IT Demo folder into the Macintosh Trash.
Although the demo program should be intuitive, these instructions only touch upon many of the features of the software. If you have any questions regarding the use of this demo program or any of the UN‑SCAN‑IT or UN‑SCAN‑IT gel features, please let us know.
P.O. Box 533 Orem, Utah 84059 USA | Tel: 1-801-377-6978 | Fax: 1-801-228-2448 | Email: info@silkscientific.com
Copyright © 2010 Silk Scientific, Inc. All rights reserved.
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